Different Responses in Induction of Allergen Specific Immunoglobulin G4 and IgE-Blocking Factors for Three Mite Subcutaneous Immunotherapy Products.
10.3349/ymj.2016.57.6.1427
- Author:
Kyung Hee PARK
1
;
Sang Chul LEE
;
Young Woong SON
;
Kyoung Yong JEONG
;
Yoo Seob SHIN
;
Jung U SHIN
;
Da Woon SIM
;
Hye Jung PARK
;
Jae Hyun LEE
;
Kwang Hoon LEE
;
Jung Won PARK
Author Information
1. Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. parkjw@yuhs.ac
- Publication Type:Comparative Study ; Original Article
- Keywords:
Blocking factor;
house dust mite;
IgG4;
immunotherapy;
specific IgE
- MeSH:
Dermatophagoides farinae;
Enzyme-Linked Immunosorbent Assay;
Humans;
Immunoglobulin E;
Immunoglobulin G;
Immunoglobulins*;
Immunotherapy*;
Indicators and Reagents;
Mites*;
Pyroglyphidae;
Sensitivity and Specificity;
Tyrosine
- From:Yonsei Medical Journal
2016;57(6):1427-1434
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Specific immunoglobulin G4 (sIgG4) and immunoglobulin E (IgE)-blocking factors produced by subcutaneous immunotherapy (SCIT) play a critical role in the induction of allergen tolerance. However, comparative studies of available SCIT reagents on the induction of sIgG4 are limited. We compared increases in sIgG4 for three different house dust mite (HDM) SCIT reagents. MATERIALS AND METHODS: Seventy-two HDM sensitized allergic patients were enrolled and classified into four groups: 1) control (n=27), 2) SCIT with Hollister-Stier® (n=19), 3) Tyrosine S® (n=16), and 4) Novo-Helisen® (n=10). Levels of specific IgE (sIgE), sIgG4, and IgE blocking factor to Dermatophagoides farinae (D. farinae) were measured using ImmunoCAP (sIgE, sIgG4) and enzyme-linked immunosorbent assay (ELISA) (IgE-blocking factors). Levels were measured before and 13.9±6.6 months after the SCIT. The allergen specificity and the induction levels of sIgE and sIgG4 were confirmed by immunoblot analysis. RESULTS: After SCIT, sIgG4 levels to D. farinae increased significantly; however, the increases differed significantly among the SCIT groups (p<0.001). Specific IgG4 levels to D. farinae were highest in Hollister-Stier® (3.7±4.1 mg/L), followed by Novo-Helisen® (2.2±2.3 mg/L) and Tyrosine S® (0.7±0.5 mg/L). In addition, patients who were administered using Hollister-Stier® showed the most significant decrease in IgE/IgG4 ratio (p<0.001) and increase in blocking factor (p=0.009). Finally, according to IgE immunoblot results, the Hollister-Stier® group showed the most significant attenuation of IgE binding patterns among others. CONCLUSION: Currently available SCIT reagents induce different levels of specific IgG4, IgE/IgG4 ratio, and IgE-blocking factor.
