Expression of pRb, p53, p16 and Cyclin D1 and Their Clinical Implications in Urothelial Carcinoma.
10.3346/jkms.2010.25.10.1449
- Author:
Kyungji LEE
1
;
Eun Sun JUNG
;
Young Jin CHOI
;
Kyo Young LEE
;
Ahwon LEE
Author Information
1. Department of Hospital Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea. klee@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
p53;
pRb;
p16;
Cyclin D1;
Urothelial Carcinoma
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Carcinoma, Transitional Cell/*metabolism/mortality/pathology;
Cyclin D1/*metabolism;
Cyclin-Dependent Kinase Inhibitor p16/*metabolism;
Female;
Humans;
Immunohistochemistry;
Lymphatic Metastasis;
Male;
Middle Aged;
Multivariate Analysis;
Neoplasm Staging;
Prognosis;
Recurrence;
Retinoblastoma Protein/*metabolism;
Survival Rate;
Tumor Suppressor Protein p53/*metabolism;
Urinary Bladder Neoplasms/*metabolism/mortality/pathology
- From:Journal of Korean Medical Science
2010;25(10):1449-1455
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to assess immunohistochemical expression of p53, pRb, p16, and cyclin D1, alone or in combination, as prognostic indicators and to investigate their correlation with clinocopathologic features of urothelial carcinoma. Immunohistochemical staining for p53, pRb, p16, and cyclin D1 was performed on a tissue microarray from 103 patients with urothelial carcinoma who underwent radical cystectomy. Of the patient samples analyzed, 36 (35%), 61 (59%), 47 (46%) and 30 (29%) had altered expression of p53, pRb, p16, and cyclin D1, respectively. Abnormal expression of p53 and pRb correlated with depth of invasion (P=0.040 and P=0.044, respectively). Cyclin D1 expression was associated with tumor stage and recurrence (P=0.017 and P=0.036, respectively). Altered pRb was significantly correlated with overall survival (P=0.040). According to the expression pattern of pRb and p53, p53/pRb (altered/normal) had worse survival than p53/pRb (normal/altered) (P=0.022). Alteration of all markers had worse survival than all normal (P=0.029). As determined by multivariate analysis, tumor stage, lymph node metastasis and the combined expression of p53 and pRb are independent prognostic factors. In conclusion, immunohistochemical evaluation of cell cycle regulators, especially the p53/pRb combination, might be useful in planning appropriate treatment strategies.
