GD3 Accumulation in Cell Surface Lipid Rafts Prior to Mitochondrial Targeting Contributes to Amyloid-beta-induced Apoptosis.
10.3346/jkms.2010.25.10.1492
- Author:
Jong Kook KIM
1
;
Sang Ho KIM
;
Hee Young CHO
;
Hee Soo SHIN
;
Hye Ryen SUNG
;
Jin Ran JUNG
;
Mei Lian QUAN
;
Dong Hong JIANG
;
Hae Rahn BAE
Author Information
1. Department of Neurology, Dong-A University College of Medicine, Medical Science Research Center, Busan, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Amyloid beta-peptide;
GD3;
Apoptosis;
Trafficking;
Lipid Rafts;
Methyl-beta-cyclodextrin
- MeSH:
Amyloid beta-Peptides/*pharmacology;
*Apoptosis;
Cell Line;
Gangliosides/*metabolism/physiology;
Humans;
Membrane Microdomains/*metabolism;
Mitochondria/*metabolism;
Sialyltransferases/genetics/metabolism;
beta-Cyclodextrins/pharmacology
- From:Journal of Korean Medical Science
2010;25(10):1492-1498
- CountryRepublic of Korea
- Language:English
-
Abstract:
Neuronal apoptosis induced by amyloid beta-peptide (A beta) plays an important role in the pathophysiology of Alzheimer's disease (AD). However, the molecular mechanism underlying A beta-induced apoptosis remains undetermined. The disialoganglioside GD3 involves ceramide-, Fas- and TNF-alpha-mediated apoptosis in lymphoid cells and hepatocytes. Although the implication of GD3 has been suggested, the precise role of GD3 in A beta-induced apoptosis is still unclear. Here, we investsigated the changes of GD3 metabolism and characterized the distribution and trafficking of GD3 during A beta-induced apoptosis using human brain-derived TE671 cells. Extracellular A beta induced apoptosis in a mitochondrial-dependent manner. GD3 level was negligible in the basal condition. However, in response to extracellular A beta, both the expression of GD3 synthase mRNA and the intracellular GD3 level were dramatically increased. Neosynthesized GD3 rapidly accumulated in cell surface lipid microdomains, and was then translocated to mitochondria to execute the apoptosis. Disruption of membrane lipid microdomains with methyl-beta-cyclodextrin significantly prevented both GD3 accumulation in cell surface and A beta-induced apoptosis. Our data suggest that rapidly accumulated GD3 in plasma membrane lipid microdomains prior to mitochondrial translocation is one of the key events in A beta-induced apoptosis.
