Recombinant Azurin from Pseudomonas aeruginosa Induces Apoptotic Cell Death in Oral Squamous Carcinoma Cells.
- Author:
Uk Kyu KIM
1
;
Hyun Jun JEON
;
Moo Hyung LEE
;
Gyoo Cheon KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan 626-870, Korea.
- Publication Type:Original Article
- Keywords:
Pseudomonas aeruginosa;
azurin;
oral squamous carcinoma;
apoptosis
- MeSH:
Antineoplastic Agents;
Apoptosis;
Azurin;
Bacteria;
Bacterial Proteins;
Carcinoma, Squamous Cell;
Cell Death;
Chromatin;
Humans;
Molecular Weight;
Mouth Neoplasms;
Pseudomonas;
Pseudomonas aeruginosa
- From:International Journal of Oral Biology
2010;35(2):35-42
- CountryRepublic of Korea
- Language:English
-
Abstract:
The use of bacteria in the treatment of cancer has a long and interesting history. The use of live bacteria in this way however has a number of potential problems including toxicity. Purified low molecular weight bacterial proteins have therefore been tested as anticancer agents to avoid such complications. Oral cancer is a widely occurring disease around the world and these lesions are typically very resistant to anticancer agents. In our present study we investigated the effects of purified recombinant azurin from Pseudomonas (P.) aeruginosa against YD-9 (p53-positive) human oral squamous carcinoma cells. Azurin showed cytotoxic effects against these cells in a dose dependent manner. The cell death accompanied by this treatment was found to be characterized by chromatin condensation and apoptotic bodies. Azurin treatment was further found to increase the expression of p53 The stabilization of p53 and induction of apoptosis in YD-9 cells by azurin suggests that it has potentially very strong anticancer properties in oral squamous carcinoma.
