Immunohistochemical Study of Transforming Growth Factor-beta2(TGF-beta2) and Vascular Endothelial Growth Factor(VEGF) after Laser Photocoagulation in the Ocular Tissues of White Rats.
- Author:
Seon Hee KIM
1
;
Shin Dong KIM
Author Information
1. Department of Ophthalmology, College of Medicine, Kosin University, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
Laser photocoagulation;
Neovascularization;
Transforming growth factor-beta2(TGF-beta2);
Vascular endothelial growth factor(VEGF)
- MeSH:
Animals;
Argon;
Diabetic Retinopathy;
Light Coagulation*;
Peroxidase;
Rats*;
Retina;
Retinal Vein Occlusion;
Retinaldehyde;
Retinopathy of Prematurity;
Transforming Growth Factor beta2;
Vascular Endothelial Growth Factor A
- From:Journal of the Korean Ophthalmological Society
1997;38(7):1162-1171
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To explain a part of the machanism of regression of new vessels after laser photocoagulation in retinal vascular occlusive diseases causing neovascularization, particularly for diabetic retinopathy, retinal vein occlusion, retinopathy of prematurity, and age-related macular degene- ration, we performed this study. Photocoagulation was done with argon green laser on the right superofrontal retina of the 15 eyes of white rats. On the first, third, and sixth day after laser photocoagulation, both eyes of 5 rats were enucleated and stained with polyclonal anti-transforming growth factor-beta2(Anti-TGF-beta 2) and anti-vascular endothelial growth factor(Anti-VEGF) antibody by immuno peroxidase technique. TGF-beta2 and VEGF were not expressed in retina of normal control eyes. After laser photocoagulation, degree of expression of TGF-beta2 and VEGF increased in 24 hours, only in and adjacent to the photocoagulated area. Thereafter, degree of expression of both factor decreased, especially that of VEGF decreased much more than that of TGF-beta2. To elucidate the exact mechanism, more qualitative and quantitative analysis will be necessary.
