Sequence Analysis and Potential Action of Eukaryotic Type Protein Kinase from Streptomyces coelicolor A3(2).
- Author:
Daisy R ROY
1
;
Sathees B C CHANDRA
Author Information
1. Department of Biological, Chemical and Physical Sciences, Roosevelt University, Chicago IL-60605 USA. schandra@roosevelt.edu
- Publication Type:Original Article
- Keywords:
eukaryotic type protein;
evolution;
protein kinase C;
Streptomyces coelicolor
- MeSH:
Anti-Bacterial Agents;
Apoptosis;
Bacteria;
Computational Biology;
Genome;
Humans;
Hydrolysis;
Isoenzymes;
Negotiating;
Phosphotransferases;
Protein Kinase C;
Protein Kinases;
Sequence Alignment;
Sequence Analysis;
Streptomyces;
Streptomyces coelicolor;
Threonine
- From:Genomics & Informatics
2008;6(1):44-49
- CountryRepublic of Korea
- Language:English
-
Abstract:
Protein kinase C (PKC) is a family of kinases involved in the transduction of cellular signals that promote lipid hydrolysis. PKC plays a pivotal role in mediating cellular responses to extracellular stimuli involved in proliferation, differentiation and apoptosis. Comparative analysis of the PKC-alpha, beta, epsilon isozymes of 200 recently sequenced microbial genomes was carried out using variety of bioinformatics tools. Diversity and evolution of PKC was determined by sequence alignment. The ser/thr protein kinases of Streptomyces coelicolor A3 (2), is the only bacteria to show sequence alignment score greater than 30% with all the three PKC isotypes in the sequence alignment. S.coelicolor is the subject of our interest because it is notable for the production of pharmaceutically useful compounds including anti-tumor agents, immunosupressants and over two-thirds of all natural antibiotics currently available. The comparative analysis of three human isotypes of PKC and Serine/threonine protein kinase of S.coelicolor was carried out and possible mechanism of action of PKC was derived. Our analysis indicates that Serine/ threonine protein kinase from S. coelicolor can be a good candidate for potent anti-tumor agent. The presence of three representative isotypes of the PKC super family in this organism helps us to understand the mechanism of PKC from evolutionary perspective.
