Effects of Topical Tamoxifen on Wound Healing of Burned Skin in Rats.
10.5999/aps.2017.44.5.378
- Author:
Shaban MEHRVARZ
1
;
Ali EBRAHIMI
;
Hedayat SAHRAEI
;
Mohammad Hasan BAGHERI
;
Sima FAZILI
;
Shahram MANOOCHEHRY
;
Hamid Reza RASOULI
Author Information
1. Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. shahram.manoochehry@yahoo.com
- Publication Type:Original Article
- Keywords:
Tamoxifen;
Wound healing;
Burns;
Keloid;
Rats
- MeSH:
Animals;
Burns*;
Cicatrix;
Cicatrix, Hypertrophic;
Contracture;
Humans;
Keloid;
Male;
Rats*;
Rats, Wistar;
Skin*;
Tamoxifen*;
Wound Healing*;
Wounds and Injuries*
- From:Archives of Plastic Surgery
2017;44(5):378-383
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: This study aimed to assess the effects of the topical application of tamoxifen on wound healing of burned skin in Wistar rats by evaluating 3 healing characteristics: fibrotic tissue thickness (FTT), scar surface area (SSA), and angiogenesis in the healed scar tissue. METHODS: Eighteen male Wistar rats were used in this study. A third-degree burn wound was made on the shaved animals’ back, measuring 2×2×2 cm. In the first group, a 2% tamoxifen ointment was applied to the wound twice daily for 8 weeks. The second group received a placebo ointment during the same period. The third group did not receive any treatment and served as the control group. RESULTS: The median (interquartile range=[Q1, Q3]) FTT was 1.35 (1.15, 1.62) mm, 1.00 (0.95, 1.02) mm, and 1.25 (0.8, 1.5) mm in the control, tamoxifen, and placebo groups, respectively (P=0.069). However, the FTT in the tamoxifen group was less than in the placebo and control groups. The median angiogenesis was 3.5 (3.00, 6.25), 8.00 (6.75, 9.25), and 7.00 (5.50, 8.25) vessels per high-power field for the control, tamoxifen, and placebo groups, respectively (P=0.067). However, the median angiogenesis was higher in the tamoxifen group than in the control group. No significant difference was observed in the mean SSA between the tamoxifen group and the control group (P=0.990). CONCLUSIONS: Local application of tamoxifen increased angiogenesis and decreased the FTT, with no change in the SSA in burned skin areas. These effects are expected to expedite the wound healing process, reducing contracture and preventing hypertrophic scar and keloid formation.
