Comparison of the Sensitivity of indirect Immunofluorescence and Immunoblot Analysis for the Detection of Autoantibodies in Pemphigus.
- Author:
Seong Jai JEONG
1
;
Chang Woo LEE
Author Information
1. Department of Dermatology, Hanyang University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Pemphigus vulgaris;
Pemphigus foliaceus;
Indirect immunofluorescence;
Immunoblot;
Sensitivity
- MeSH:
Alkaline Phosphatase;
Autoantibodies*;
Diagnosis;
Fluorescent Antibody Technique, Indirect*;
Humans;
Immunoglobulin G;
Pemphigus*;
Skin Diseases, Vesiculobullous
- From:Korean Journal of Dermatology
1996;34(2):212-218
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Immunolcgical assays are required for the accurate diagnosis of autoimmune bullous dermatoses including pemphigus vulgaris(PV) and pemphigus foliaceus(PF). In the detection of circulating autoantibodies to pemphigus antigens(desmosomal components), the priority remains controversial between indirect immunofluorescence(IF) and immunoblot(IB) assay. OBJECTIVE: In the present study we compared the sensitivity of indirect IF and that of IB using amplified alkaline phosphatase staining system in the detection of pemphigus autoantibodies. PATIENTS: We selected eight patients with serum endpoint titer of 1:80 in preliminary study. Among these patients three were PV and five were PF. METHODS/RESULTS: The titers of IgG autoantibodies found on indirect IF were confirmed as 1: 80 in all patients, whereas the titers examined by IB assay were much higher, 1: 640 to 1: 2560. In the 3 sera of PV patients, the titers of two cases were 1: 1280 and the third case was 1: 2560. In 5 cases of PF, one was 1:640, two were 1: 1280, and two were 1:2560. CONCLUSION: This result suggests that the immunoblot examination using amplified alkaline phosphatase staining system demonstrates higher sensitivity compared with indirect IF(p=0.0003 by Mann-Whitney U test) in the detection of pemphigus autoantibodies.
