Detection and Typing of Herpes Simplex Virus by Polymerase Chain Reaction.
- Author:
Yong Hyun KIM
1
;
Jae Bok JUN
;
Do Won KIM
;
Sang Lip CHUNG
;
Jung Chul KIM
Author Information
1. Department of Dermatology, Kyungpook National University School of Medicine, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
Herpes simplex virus;
Polymerase chain reaction;
Restriction analysis
- MeSH:
Dermatology;
Diagnosis;
DNA;
Genome;
Herpes Simplex*;
Herpesvirus 1, Human;
Herpesvirus 2, Human;
Herpesvirus 3, Human;
Humans;
Incidence;
Kaposi Varicelliform Eruption;
Polymerase Chain Reaction*;
Polymorphism, Restriction Fragment Length;
Prevalence;
Prognosis;
Simplexvirus*;
Umbilicus
- From:Korean Journal of Dermatology
1996;34(2):248-256
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Herpes simplex virus(HSV) infections are very common viral illnesses in dermatology and they have shown a tendency to increase in prevalence and incidence, and they would seem to have become more prevalent recently. This has resulted in an increased need for the rapid diagnosis of these infections. It has also become important to recognize the types of HSV in the clinical setting, because the two types differ in their natural histories and prognoses. OBJECTIVE: The purpose of this study is to detect and type HSV DNA by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). We investigated the relationship between the clinical manifestations and type using PCR. METHODS: The study population consisted of 40 cases of HSV infections and 10 cases of varicella-zoster virus infections as negative controls. We used a pair of primers designated by Sakaoka et. al and performed restriction analysis after PCR. RESULTS: No specific amplification was observed using the varicella-zoster virus. A total of 40 patients were examined by PCR and 28 were positive. Of 17 patients with lesions which developed above the umbilicus, l0 were positive. Out of 8 patients with lesions below the umbilicus, 5 were positive. Virus genomes were detected in the 13 out of 15 patients with eczema herpeticum. CONCLUSION: The method used in this study is useful in differentiating HSV-1 from HSV-2 in fections. These data suggest that the PCR results were nearly consistent with the clinical manifestations.
