Expression of Epidermal Protein Antigens and Basement Membrane Components in Human Epidermis Reconstructed by Culture.
- Author:
Kwang Hyun CHO
1
;
Dong Youn LEE
;
Hee Chul EUN
;
Kyoung Chan PARK
;
Jai Il YOUN
Author Information
1. Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Epidermal protein antigens;
Basement membrane components
- MeSH:
Antibodies;
Antigens, Differentiation;
Basement Membrane*;
Collagen Type IV;
Dermis;
Eosine Yellowish-(YS);
Epidermis*;
Hematoxylin;
Humans*;
Keratin-1;
Keratin-13;
Keratinocytes;
Laminin;
Mass Screening;
Parents;
Proliferating Cell Nuclear Antigen;
Receptor, Epidermal Growth Factor;
Skin
- From:Korean Journal of Dermatology
1996;34(2):264-272
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: A number of in vitro skin models have been developed for the purpose of the screening of cosmetics, pharmaceuticals, and environmental chemicals. To mimic the skin in vivo, a model should resemble morphologically and biochemically the parent, tissue. OBJECTIVE: The purpos of this study is to study the differentiation and organization of the artificial epidermis in comparsion with epidermis in vivo based on the expression of epidermal protein antigens and basement membrane components. METHODS: Human keratinocytes were cultured on deepidermidized dermis (RE-DED) or on fibroblast-populated collag-,n matrix (LSE). After 10 days culture, the sections of RE-DED and LSE were stained with hematoxylin and eosin. An immunohistochemical study was also performed with the sections of RE-DED and LSE using antibodies recognizing proliferating cell nuclear antigens (PCNA), epidermal growth factor receptor (EGFR), keratin 1, involucrin, filaggrin, loricrin, keratin 13, type IV collagen, and laminin. RESULTS: In both culture systems(RE-DED and LSE) a multilayered epidermis with a horny layer was observed. In the human epidermis reconstructed by both culture systems, differentiation markers appeared but with a topography slightly different from that of epidermis in vivo, and components of the basement membrane was also expressed. CONCLUSION: Our findings suggest the epidermis obtained in both culture systems(RE-DED and LSE) resembled in vivo epidermis morphologically and biochemically, although it was not the same.
