Isolation and Characterization of Reovirus in Korea.
- Author:
Ki Joon SONG
1
;
Byung Chul KANG
;
Young Eun LEE
;
Luck Ju BAEK
;
Yong Ju LEE
;
Jin Won SONG
Author Information
1. Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 136-705, Korea.
- Publication Type:Original Article
- Keywords:
Reovirus;
S4 segment;
Isolation;
Hemagglutination inhibition test
- MeSH:
Animals;
Genome;
Hemagglutination Inhibition Tests;
Humans;
Korea*;
Lung;
Mice;
Phylogeny;
RNA, Double-Stranded
- From:Journal of the Korean Society of Virology
1999;29(2):65-74
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Reovirus was found to inhabit both the respiratory and the enteric tract of human find animals. The genome of reovirus comprises 10 segments of double-stranded RNA, total size 24 kbp. Nine strains of reovirus were isolated from human and field mice in Korea. Aseptically collected sera from human and lung tissues from field mice were used for virus isolation For serotype determination, hemagglutination inhibition test was used, and three strains were confirmed to type 2 and six strains to type 3. To determine the genomic diversity and molecular phylogeny of reoviruses isolated in Korea, part of 54 genomic segment of reovirus was enzymatically amplified and directly sequenced. In nucleotide level, Apo98-35 strain showed 15.4%, 19.3%, and 14.4% differences compared to type 1 (T1L, Lang), type 2 (T2J), and type 3 reference strains, respectively. In amino acid level, Apo98-35 strain showed 10.5%, 13.7%, and 9.5% differences compared to type 1, type 2, and type 3 reference strains, respectively. Using the maximum parsimony method based on 285 bp spaning region of the S4 genomic segment, phylogenetic analysis indicated that Apo98-35 from Korea formed different phylogenetic branch. Our data obtained by sequence and phylogenetic analyses of reoviruses are consistent with the distinct geographically dependent evolution of reoviruses in Korea.
