Detection of Glycoproteins (B and D) and Ttymidine Kinase Genes of Herpes simplex virus Type 2 Strain G.
- Author:
Hyun KANG
1
;
Jong Kuk PARK
;
Hong Sun UH
;
Soo Young KIM
;
Hyung Hoan LEE
Author Information
1. Department of Biological Science, Konkuk University, Seoul 143-701, Korea. hhlee@kkucc.konkuk.ac.kr
- Publication Type:Original Article
- Keywords:
Herpes simplex virus;
Glycoprotein;
Glycoproteins B and D genes;
Thymidine kinase gene;
TK protein
- MeSH:
Blotting, Southern;
Clone Cells;
DNA;
Genomic Library;
Glycoproteins*;
Herpes Simplex*;
Herpesvirus 2, Human*;
Phosphotransferases*;
Plasmids;
Simplexvirus*
- From:Journal of the Korean Society of Virology
1999;29(2):99-105
- CountryRepublic of Korea
- Language:English
-
Abstract:
BamHI restriction patters and genomic library of Herpes simplex virus type 2 (HSV-2) stram G were constructed, and locations of the glycoproteins gB and gD, and it genes on the fragments were detected by Southern blot analysis. HISV-2 genomic DNAs were cleaved into twenty-seven fragments by BamHI enzyme in the range of 0.72 to 15.08 (total 150.44 kb), which were cloned into the BamHI site of pBluescript SK(+) to construct genome library of the HSV-2. The library was named by the order of the fragment size from smallest one to largest one. HSV-2 glycoprotein gD gene was located in PHLA2-21 and PHLA2-22 recombinant plasmids, gB gene in PHLA2-24 plasmic, and it gene in PHLA2-11 clone by Southern blot analysis.
