Effects of Sinusoidal Electromagnetic Field on Structure and Function of Different Kinds of Cell Lines.
10.3349/ymj.2006.47.6.852
- Author:
Ah Ram SUL
1
;
Si Nae PARK
;
Hwal SUH
Author Information
1. Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea. hwal@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Electromagnetic field;
fetal osteoblast;
aortic vascular smooth muscle cell;
B lymphoblast;
cortical neuronal cell;
proliferation
- MeSH:
Signal Transduction;
Microfilaments/radiation effects;
Humans;
Gap Junctions/metabolism/radiation effects;
*Electromagnetic Fields;
Cell Proliferation/radiation effects;
Cell Physiology/*radiation effects;
Cell Line;
Cell Differentiation/radiation effects;
Cell Cycle/radiation effects
- From:Yonsei Medical Journal
2006;47(6):852-861
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study investigated that whether a 2 mT, 60 Hz, sinusoidal electromagnetic field (EMF) alters the structure and function of cells. This research compared the effects of EMF on four kinds of cell lines: hFOB 1.19 (fetal osteoblast), T/G HA-VSMC (aortic vascular smooth muscle cell), RPMI 7666 (B lymphoblast), and HCN-2 (cortical neuronal cell). Over 14 days, cells were exposed to EMF for 1, 3, or 6 hours per day (hrs/d). The results pointed to a cell type-specific reaction to EMF exposure. In addition, the cellular responses were dependent on duration of EMF exposure. In the present study, cell proliferation was the trait most sensitive to EMF. EMF treatment promoted growth of hFOB 1.19 and HCN-2 compared with control cells at 7 and 14 days of incubation. When the exposure time was 3 hrs/d, EMF enhanced the proliferation of RPMI 7666 but inhibited that of T/G HA- VSMC. On the other hand, the effects of EMF on cell cycle distribution, cell differentiation, and actin distribution were unclear. Furthermore, we hardly found any correlation between EMF exposure and gap junctional intercellular communication in hFOB 1.19. This study revealed that EMF might serve as a potential tool for manipulating cell proliferation.
