Purification and preliminary studies for application of lectin from haemolymph clam (Meretrix meretrix Linne) in medicine.
- Author:
Nguyen Dang Cao
- Publication Type:Journal Article
- Keywords:
lectin
- MeSH:
isolation & purification;
Lectins;
medicine
- From:Journal of Medical and Pharmaceutical Information
1998;(1):34-38
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
A lectin from the haemolymph clam was purified by molecular sieving on sephadex- G75 column. The products have a high purity. The purity of lectin was assessed by SDS polyacrylamide gel. Two doublets band of apparent molecular weights of respectively about 14,000-17,000 and 20,000-23,000 Dalton were revealed upon staining with Coomassie blue. Optimum-pH of the lectin from the clams is pH 6,5-7,0 and 8,5-9,0. The lectins of the clams had very different specificity for hydratecarbons and glucoproteins