The Effect of VEGF (Vascular endothelial growth factor) on Endothelial Cells in Pathogenesis of Preeclampsia.
- Author:
Yong Kyoon CHO
1
;
Hong Kyoon LEE
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Inje University, Sanggye Paik Hospital, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Preeclampsia;
endothelial dysfunction;
VEGF;
prostacyclin
- MeSH:
Endothelial Cells*;
Endothelium;
Epoprostenol;
Female;
Human Umbilical Vein Endothelial Cells;
Humans;
Immunoenzyme Techniques;
Pre-Eclampsia*;
Pregnant Women;
Vascular Endothelial Growth Factor A*
- From:Korean Journal of Obstetrics and Gynecology
2002;45(8):1354-1359
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Preeclampsia is primarily a disorder of the maternal endothelium. The objective of this study was to assess the effect of VEGF on human umbilical vein endothelial cells (HUVEC) in vitro. METHODS: The serum concentrations of VEGF in women with preeclampsia and normotensive pregnant women were measured with a sensitive and specific enzyme immunoassays. After HUVEC were isolated and cultured in vitro, these HUVEC were stimulated with VEGF, sera from women with preeclampsia and normotensive pregnant women for 24 hours. And then prostacyclin levels were measured. RESULTS: The serum concentrations of VEGF were significantly higher in women with preeclampsia than in normotensive pregnant women. Vascular endothelial growth factor induced a significant concentration- dependent increase in prostacyclin production. HUVEC stimulated with sera from women with preeclampsia showed an increase in production of prostacyclin. VEGF concentration in serum was correlated with prostacyclin production by the stimulated cells. CONCLUSION: VEGF may be one of the circulating factors that cause the alteration in endothelial function and the high serum concentration of VEGF is thought to be important in the pathogenesis of preeclampsia.
