The Factors Affecting Transplanted Hepatocytes Repopulation in Rats with Liver Fibrosis.
- Author:
Sang Woo LEE
1
;
Seon Ok MIN
;
Sae Byeol CHOI
;
Kyung Sik KIM
Author Information
1. Department of Surgery, Yonsei University College of Medicine, Korea. kskim88@yuhs.ac
- Publication Type:Original Article
- Keywords:
Hepatocyte transplantation;
Engraftment;
Liver fibrosis;
Kupffer cell;
Analbuminemic rat (NAR)
- MeSH:
Animals;
Cell Transplantation;
Doxorubicin;
Drinking Water;
End Stage Liver Disease;
Fibrosis;
Gadolinium;
Gliotoxin;
Hepatocytes;
Humans;
Kupffer Cells;
Liver;
Liver Cirrhosis;
Perfusion;
Rats;
Serum Albumin;
Thioacetamide;
Tissue Therapy;
Transplants
- From:Korean Journal of Hepato-Biliary-Pancreatic Surgery
2009;13(4):189-197
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Cell therapy for various diseases has gained wide acceptance. Because most patients with chronic liver failure have mild-to-severe liver cirrhosis, there are many limitations to clinical applications. We analyzed how to increase cell engraftment in rats with liver fibrosis. METHODS: We used analbuminemic SD rats (NARs) as recipients of syngeneic CAG-EGFP SD hepatocytes obtained by the 2 perfusion method. Hepatic fibrosis was induced with thioacetamide in drinking water for 6 weeks in the recipient NARs. NARs were pre-treated with gadolinium, doxorubicin, and gliotoxin before hepatocyte transplantation. We evaluated the degree of cell engraftment by RT-PCR and immunofluorescent staining for GFP and albumin. The transplanted cells were detected by immunostaining for albumin, and serum albumin was also measured. RESULTS: Although detection of GFP by RT-PCR was variable, albumin was detected in all groups 4 wks after hepatocyte transplantation. GFP and albumin were also detected by immunofluorescent staining 1 and 4 wks after cell transplantation. In control rats, albumin production was maximal at 3 wks, and after that it rapidly decreased. In the gadolinium and doxorubicin-treated group, albumin production was increased up to 4 wks. Albumin production in the gadolinium-treated group was superior to that of the doxorubicin-treated group. CONCLUSION: Kupffer cells play the most important role in cell engraftment in hepatic fibrosis. Therefore, perturbation of kupffer cells in hepatic fibrosis is needed to increase cell engraftment.
