Evaluation of Anti-allergic Effect of Bifidobacteria in Murine Model of Peanut Allergy.
- Author:
Soo Young LEE
1
;
Se Ko OH
;
Seok Won PARK
;
Gey Ree JEON
;
Ji Yun KIM
;
So Yoon YOON
;
Geun Ek JI
Author Information
1. Department of Pediatrics, Ajou University School of Medicine, Suwon, Korea. jsjs87@ajou.ac.kr
- Publication Type:Original Article
- Keywords:
Murine model;
Peanut allergy;
Bifidobacteria;
IgE;
Cytokine
- MeSH:
Animals;
Cholera Toxin;
Immunoglobulin E;
Interleukin-12;
Interleukin-4;
Mice;
Peanut Hypersensitivity*
- From:Pediatric Allergy and Respiratory Disease
2006;16(2):131-141
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We underwent this study to evaluate the immunomodulating effects of intragastric administration of bifidobacterium(BGN4) using murine model of peanut allergy. METHODS: C3H/HeJ mice were sensitized with 1 mg/dose of crude peanut(PN) extract with cholera toxin, intragastricly. Group I mice were pretreated with BGN4 for 14 days before PN snesitization, Group II were treated 14-days each before and during sensitization, Group III were sham treated PN sensitized mice, and Group IV were naive. PN-specific serum IgE levels and PN stimulated cytokine productions from splenocyte were measured in study groups. RESULTS: PN-specific IgE levels were significantly lowered in Group II mice compare to Group I or Group III. PN-stimulated IL-4 productions were also remarkably depressed in Group II mice. The ratio of IFN-gamma/IL-4 in Group II was the highest among experimental Groups, furthermore, PN-stimulated IL-12 production was only measured in Group II mice. The decreased levels of PN-specific IgE in Group II consist with decreased production of IL-4 and increased ratio of IFN-gamma/IL-4 in this experiment. CONCLUSION: BGN4 treatment, especially pre-and-during PN sensitization, seemed to have anti-allergic effect by suppressing PN-specific IgE production. And lowered production of IL-4, increased production of IL-12, and the increased ratio of IFN-gamma/IL-4 could be suggested as a part of immunomodulating mechanism of BGN4 treatment in this experiment.
