Microbial Genome Analysis and Application to Clinical Bateriology.
10.12701/yujm.2002.19.1.1
- Author:
Sung Kwang KIM
1
Author Information
1. Department of Microbiology College of Medicine, Yeungnam University, Daegu, Korea.
- Publication Type:Comparative Study ; Review
- Keywords:
Prokaryotic organism 16S rRNA;
Sequence analysis;
DNA-DNA reassociation
- MeSH:
Bacteriology;
DNA;
Genome, Microbial*;
Sequence Analysis
- From:Yeungnam University Journal of Medicine
2002;19(1):1-10
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
With the establishment of rapid sequence analysis of 16S rRNA and the recognition of its patential to determine the phylogenetic position of any prokaryotic organism, the role of 16S rRNA similarities in the present species definition in bacteriology need to be clarified. Comparative studies clearly reveal the limitations of the sequence analysis of this conserved gene and gene product in the determination of relationship at the pathogenic strain level for which DNA-DNA reassociation exprements still constitute the superior method. Since today the primary structure of 16S rRNA is easier to determine than hybridization between DNA strands, the strength of the sequence analysis is to recognize the level at which DNA pairing studies need to be performed, which certainly applies to similarities of 97% and higher.
