High fructose and high fat diet increased bone volume of trabecular and cortical bone in growing female rats.
10.4163/jnh.2015.48.5.381
- Author:
Hyejin AHN
1
;
Sooyeon YOO
;
Yoo Kyoung PARK
Author Information
1. Department of Medical Nutrition, Kyung Hee University, Gyeonggi 17104, Korea. ypark@khu.ac.kr
- Publication Type:Original Article
- Keywords:
high-fructose diet;
high-fat diet;
kidney function;
bone growth;
growing rats
- MeSH:
Animals;
Body Weight;
Bone Development;
Diet;
Diet, High-Fat*;
Energy Intake;
Female*;
Fructose*;
Humans;
Kidney;
Osteocalcin;
Rats*;
Tibia;
Weight Gain
- From:Journal of Nutrition and Health
2015;48(5):381-389
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The objective of this study was to investigate the effects of a high fructose and fat diet on bone growth and maturation in growing female rats. METHODS: Three-week-old female SD rats were randomly assigned to four experimental groups; the control group (CON: fed control diet based on AIN-93G, n = 8); the high-fructose diet group (HFrc: fed control diet with 30% fructose, n = 8); the high-fat diet group (Hfat: fed control diet with 45 kcal% fat, n = 8); and the high-fat diet plus high fructose group (HFrc + HFat: fed diets 45 kcal% fat with 30% fructose, n = 8). Each group was assigned their respective diets for the remaining eight weeks. Bone-related parameters (bone mineral density (BMD) and structural parameters, osteocalcin (OC), deoxypyridinoline (DPD)) and morphologic changes of kidney were analyzed at the end of the experiment. RESULTS: Final body weights and weight gain were higher in the HFat and HFrc + HFat groups and showed higher tendency in the HFrc group compared with those of the CON group (p < 0.05); however, no significant difference in caloric intake was observed among the four experimental groups. The serum OC levels of the HFrc and HFrc + HFat groups were lower than those of the CON and HFat groups (p < 0.05). Urinary levels of DPD did not differ among the experimental groups. BV/TV and Tb.N of trabecular bone were higher in the HFrc + HFat group and showed a higher tendency in the HFrc group than those of the CON and HFat groups (p < 0.05). Tb.Pf of trabecular bone were lower in the HFrc + HFat group than those in the CON and HFat groups (p < 0.05). However, no difference in trabecular BMD was observed among the experimental groups. Cortical bone volume was higher in the HFat and HFrc + HFat groups than in the CON and HFrc groups (p < 0.05). No morphology change in kidney was observed among the experimental groups. CONCLUSION: Our study suggests that 8 weeks of high-fructose and high fat intake could improve the bone quality (Structural parameters) of trabecular and cortical bone of tibia in growing female rats.
