Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell.
- Author:
Seung Geun YEO
1
;
Chang Il CHA
;
Dong Choon PARK
Author Information
- Publication Type:Original Article
- Keywords: B-1 cell; B-2 cell; immunology; differentiation; cell cycle; p21
- MeSH: Allergy and Immunology; Animals; Ascitic Fluid; B-Lymphocytes; Cell Cycle; Cell Proliferation; Enzyme-Linked Immunosorbent Assay; Immunoglobulin M; Mice; Peritoneal Cavity; Propidium; Real-Time Polymerase Chain Reaction; S Phase; Spleen
- From:Immune Network 2006;6(1):1-5
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: B cell subset has been divided into B-1 cells and B-2 cells. B-1 cells are found most prominently in the peritoneal cavity, as well as constituting a small proportion of splenic B cells and they are larger and less dense than B-2 cells in morphology. This study was designed to compare the differences in their proliferation and differentiation between B-1 and B-2 cell. METHODS: We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. Secreted IgM was measured by enzyme-linked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay. Cell cycle analysis by propidium iodide was performed. p21 expression was assessed by real time PCR. RESULTS: Cell proliferation and cell cycle progression in B-1 and B-2 cells, which did not occur in the absence of LPS, required LPS stimulation. After LPS stimulation, B-1 and B-2 cells were shifted to S and G2/M phases. p21 expression by resting B-1 cells was higher than that of resting B-2 cells. CONCLUSION: B-1 cells differ from conventional B-2 cells in proliferation, differentiation and cell cycle.
