Comparison between L and E gene amplification analytical methods for human papillomavirus typing.
10.3802/jgo.2008.19.4.251
- Author:
Hong Bum CHO
1
;
Young Jae KIM
;
Kyung Tai KIM
Author Information
1. Department of Biological Engineering, SeoKyeong University, Korea.
- Publication Type:Original Article
- Keywords:
Human papillomavirus;
L1 gene;
E6/E7 gene
- MeSH:
Gene Amplification;
Humans;
Light;
Membranes;
Polymerase Chain Reaction
- From:Journal of Gynecologic Oncology
2008;19(4):251-255
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: L and E6/E7 gene amplification analyses were compared to identify human papillomavirus (HPV) infection and verify the HPV type, with the intent to minimize HPV typing errors. METHODS: L1 gene verified HPV typing was accomplished via polymerase chain reaction (PCR) and membrane assays. Verification of HPV typing via E6/E7 genes was accomplished through nested multiplexed PCR. The results from 104 samples were compared. RESULTS: The rates of accordance and difference were 35% and 65%, respectively. For 29% of the analyses, nested multiplexed PCR was more diversified than the membrane assay. CONCLUSION: HPV can be classified into low-risk HPV and high-risk HPV groups. In parallel amplifications of the L and E genes is more efficient for accurate diagnosis in light of the different symptoms and attendant precautions of the risk groups.
