Species Identification Using Cytochrome B Gene.
- Author:
Soong Deok LEE
1
;
Yoon Seong LEE
;
Jung Bin LEE
Author Information
1. Department of Forensic Medicine, Seoul National University College of Medicine.
- Publication Type:Original Article
- Keywords:
Species Identification;
Mitochondrial DNA;
Cytochrome B gene;
Sequence
- MeSH:
Animals;
Base Sequence;
Cytochromes b*;
Cytochromes*;
DNA;
DNA, Mitochondrial;
Humans;
Placenta;
Polymerase Chain Reaction;
Sequence Analysis
- From:Korean Journal of Legal Medicine
2001;25(1):34-39
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The feasibility of species identification using sequence analysis of the cytochrome B (Cyt B) gene in mitochondrial DNA was investigated. DNA was extracted from nine different animals that could be easily met in our surroundings and Cyt B gene was amplified. Direct sequencing results for the amplified PCR products were compared with each other. Human was also included. Nucleotide sequence of the Cyt B gene for each animals was also compared with the previously known ones registered in nucleotide databases, Genebank. The inter-species sequence variation was high as the percent similarity of each sequences ranged 64.6-83.5%. Compared to this the percent similarity of sequences obtained here were high when compared to the sequences of the same species registered in the database showing relatively low intra-species variation. This data shows that the nucleotide sequences of Cyt B gene in a certain biological materials can be identified at species level. The applicability of this method to the forensic field is also demonstrated by performing a casework; determination of the origin for the placentae which were commercial available as "invogorant". Points about the use of Cyt B gene in forensic field was also reviewed.
