Study on Causes of Respiratory Disease to the Middle East Respiratory Syndrome-negative Subjects.
10.4167/jbv.2017.47.3.156
- Author:
Su Jeong HWANG
1
;
Dong Ju PARK
;
Hee Soo KOO
;
Ho Cheol YUN
;
Pyeung Tae GU
;
Mi Ok LEE
;
Sung Hyun JIN
Author Information
1. Busan Metropolitan City Institute of Health & Environment, Busan, Korea. kies98@korea.kr
- Publication Type:Original Article
- Keywords:
Middle East respiratory syndrome coronavirus (MERS-CoV);
Human Metapneumovirus (hMPV);
Mycoplasma pneumonia (M. pneumonia);
Phylogenetic analysis
- MeSH:
Adenoviridae;
Bacteria;
Genotype;
Humans;
Korea;
Metapneumovirus;
Middle East Respiratory Syndrome Coronavirus;
Middle East*;
Paramyxoviridae Infections;
Pneumonia;
Pneumonia, Mycoplasma;
Repetitive Sequences, Nucleic Acid;
Respiratory Tract Infections;
Rhinovirus
- From:Journal of Bacteriology and Virology
2017;47(3):156-164
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe cases of human respiratory disease. The current outbreak of infection with this virus in South Korea, which began on May 20, 2015, has infected 186 patients and caused 36 deaths within 2 months. In this study, to investigate the viral pathogen causing acute respiratory infections, multiplex/RT-PCR was performed on were obtained from nucleic acid of the Middle East Respiratory Syndrome-negative subjects. Viruses and atypical bacteria were detected in 39 of 337 (11.6%). Frequent viruses were human rhinovirus (n=11, 3.3%), human metapneumovirus (n=9, 2.7%), parainfluenza (n=9, 2.7%) and adenovirus (n=4, 1.2%). Mycoplasma pneumonia (M. pneumonia) was detected in 1.8 % (n=6). Out of 9 human metapneumovirus (hMPV) positive samples, 6 samples were successfully sequenced using F gene. And M. pneumoniae was sequencing of a repetitive region of the P1 gene. Phylogenetic analysis revealed that hMPV clustered into A2b lineage (n=4), B2 lineage (n=2) and M. pneumoniae clustered into two genotypes: Type 1 (n=4), Type 2a (n=2).
