Indirect ELISA for the Detection of Rabies Virus Antibodies in Dog Sera.
10.4167/jbv.2017.47.3.148
- Author:
Dong Kun YANG
1
;
Ha Hyun KIM
;
Seung Heon LEE
;
Miryun JI
;
In Soo CHO
Author Information
1. Viral Disease Research Division, Animal and Plant Quarantine Agency, MAFRA, Gimcheon, Korea. yangdk@korea.kr
- Publication Type:Original Article
- Keywords:
Dog;
Indirect ELISA;
Rabies virus;
Sero-surveillance;
Rabies virus nucleoprotein;
Recombinant baculovirus system
- MeSH:
Animals;
Antibodies*;
Asian Continental Ancestry Group;
Baculoviridae;
Cell Culture Techniques;
Chromatography;
Dogs*;
Enzyme-Linked Immunosorbent Assay*;
Humans;
Nucleoproteins;
Rabies virus*;
Rabies*;
Sensitivity and Specificity;
Vaccination
- From:Journal of Bacteriology and Virology
2017;47(3):148-155
- CountryRepublic of Korea
- Language:English
-
Abstract:
Rabies is known as the most fatal disease in all warm-blooded animals, including dogs. Among animals that transmit rabies, dogs are mainly responsible for transmitting animal rabies in Asian countries. Detection of rabies virus (RABV) antibodies in dogs is performed by fluorescent antibody virus neutralization (FAVN) test or rapid fluorescent focus inhibition test. These standard assays are difficult to carry out in diagnostic laboratories without sufficient instruments, designated RABV, and cell culture systems. An alternative assay that is easy to conduct and time efficient is required for rapid sero-surveillance following vaccination. Recombinant baculovirus expressing RABV nucleoprotein (RVN) was constructed and the recombinant protein was purified using Ni-NTA and fast protein liquid column chromatography. We developed and evaluated an indirect enzyme-linked immunosorbent assay (I-ELISA) with recombinant RVN for the detection of RABV antibodies in 122 dog serum samples. The I-ELISA results obtained from these samples were compared with FAVN results. The sensitivity, specificity, and accuracy of I-ELISA were 88.1%, 92.5%, and 91.0%, respectively, compared with FAVN. Results of I-ELISA were significantly correlated with that of FAVN (r = 0.81). These results suggest that I-ELISA with recombinant RVN is useful for sero-surveillance of RABV in dog sera.
