Distribution of Phospholipase C Isozymes in Rat Retina: Immunohistochemical Study.
- Author:
Ji Myong YOO
1
;
Wan Sung CHOI
;
Myeong Ok KIM
;
Hea Won YOON
;
Jong Moon PARK
;
Joon Kyeong SONG
Author Information
1. Department of Ophthalmology, College of Medicine, Gyeong Sang National University, Korea.
- Publication Type:Original Article
- Keywords:
Immunohistochemistry;
PLC isozyme;
Retina
- MeSH:
Animals;
Antibodies, Monoclonal;
Ganglion Cysts;
Immunohistochemistry;
Isoenzymes*;
Nerve Fibers;
Peroxidase;
Phospholipase C beta;
Phospholipases*;
Photoreceptor Cells;
Rats*;
Retina*;
Type C Phospholipases*
- From:Journal of the Korean Ophthalmological Society
1992;33(12):1194-1199
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In order to explore the existence and distribution of phospholipase (PLC) isozymes in the rat retina, immunohistochemical staining was applied using monoclonal antibodies against PLC isozymes (PLC beta; K92, PLC gamma; D7, F7, PLC delta; R32, S11). For immunohistochemical detection, avidin-biotin peroxidase complex (ABC) method was performed on frozed tissue sections of rat retina. Our study showed that PLC isozymes have particular distributional patterns in the retina. Namely, PLC beta is broadly distributed in the outer and inner segments of photoreceptor cell layer, nuclear layer and ganglion cell layer. PLC gamma is mainly appeared in the nerve fiber layer, ganglion cell layer and inner nuclear layer. PLC delta is confined only in the ganglion cell layer. These results clearly demonstrate the PLC isozymes may have their own role in the transduction of light pathway in the retina. However, further studies will be required to verify theirs precise role in the photoreception.
