Cyclooxygenase-2 Expression and Its Prognostic Significance in Clear Cell Renal Cell Carcinoma.
10.4132/KoreanJPathol.2012.46.3.237
- Author:
Ji Won LEE
1
;
Jeong Hwan PARK
;
Ja Hee SUH
;
Kyung Han NAM
;
Ji Young CHOE
;
Hae Yoen JUNG
;
Ji Yoen CHAE
;
Kyung Chul MOON
Author Information
1. Department of Pediatrics, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Carcinoma, renal cell;
Cyclooxygenase 2;
Prognosis
- MeSH:
Humans;
Risk Factors
- From:Korean Journal of Pathology
2012;46(3):237-245
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The prognostic value of cyclooxygenase-2 (COX-2) in human renal cell carcinoma (RCC) remains unclear. The purposes of this study are to elucidate the clinical significance of COX-2 in clear cell RCC (CCRCC) and to assess the treatment effect of COX-2 inhibition on CCRCC cell lines. METHODS: Using tumor samples obtained from 137 patients who had undergone nephrectomy at Seoul National University Hospital, we evaluated COX-2 expression on immunohistochemistry. Moreover, we performed the cell proliferation assay using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) and cell invasion assay. Thus, we evaluated the effect of meloxicam, an inhibitor of COX-2, in two human CCRCC cell lines. RESULTS: Cancer-specific survival (p=0.038) and progression-free survival (p=0.031) were shorter in the COX-2 high expression group. A multivariate logistic regression model showed that COX-2 expression was an independent risk factor for pTNM stage and Fuhrman nuclear grade. The MTT assay revealed that COX-2 inhibition led to the suppression of the proliferation of CCRCC cell lines. Moreover, it also reduced their invasion capacity. CONCLUSIONS: This study postulates that COX-2 is a poor prognostic indicator in human CCRCC, suggesting that COX-2 inhibition can be a potential therapy in CCRCC.
