Study of the difference of high and low metastasis cell line's gene expression map and metastasis-related genes of adenoid cystic carcinoma.
- Author:
NaiShuo ZHU
1
;
JieLin YANG
;
YingMing WANG
;
XiaoFeng GUAN
Author Information
1. Lab of Molecular Virology & Immunology, School of Life Sciences, Fudan University Shanghai 200433.
- Publication Type:Original Article ; In Vitro ; Research Support, Non-U.S. Gov't
- Keywords:
adenoid cystic carcinoma cell line;
suppression subtractive hybridization;
tumor metastasis
- MeSH:
Blotting, Northern;
Carcinoma, Adenoid Cystic/*genetics/secondary;
*Gene Expression;
Gene Expression Profiling;
Gene Expression Regulation, Neoplastic;
Human;
In Vitro;
Molecular Sequence Data;
Neoplasm Metastasis/*genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Tumor Cells, Cultured
- From:Experimental & Molecular Medicine
2003;35(4):243-248
- CountryRepublic of Korea
- Language:English
-
Abstract:
We searched for metastasis-related genes in adenoid cystic carcinoma by suppression subtractive hybridization analysis of high and low metastasis cell lines. Twelve genes (ten previously identified and two novel sequences) were identified as being expressed at lower levels in high metastasis cell line Acc-M when compared to low metastasis cell line Acc-2. The known sequences corresponded to the genes for cysteine-rich angiogenesis induction factor (cyr61), chromosome 7 RP11-52501 clone, G-protein, WAS familial ferritin I heavy chain, jumping translocation breakpoint, eukaryotic translation elongation, folate receptor and three ribosomal proteins. Among them, the G protein and ferritin I heavy chain genes contained mutations in the high metastasis cell line. The two novel gene sequences have been named ACC metastasis-associated RNH and ACC metastasis-associated suspected protein (GenBank # AF522024 and AF522025, respectively). Taken together, these results suggest that reduced expression and/or mutation of several genes in the tumor cell line Acc-M are associated with high tumor metastasis, providing important molecular biological materials for further study of metastasis control and possible targets for cancer gene therapy.
