IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans.
- Author:
Amir Hossien RAHBARI
1
;
Hossien KESHAVARZ
;
Saeedeh SHOJAEE
;
Mehdi MOHEBALI
;
Mostafa REZAEIAN
Author Information
- Publication Type:Original Article ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords: Toxoplasma gondii; IgG avidity test; ELISA; IgM; indirect immunofluorescent antibody test
- MeSH: Antibodies, Protozoan/*blood; *Antibody Affinity; Clinical Laboratory Techniques/*methods; Enzyme-Linked Immunosorbent Assay/methods; Fluorescent Antibody Technique, Indirect; Humans; Immunoglobulin G/*blood; Immunoglobulin M/blood; Iran; Parasitology/*methods; Toxoplasmosis/*diagnosis
- From:The Korean Journal of Parasitology 2012;50(2):99-102
- CountryRepublic of Korea
- Language:English
- Abstract: Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI< or =50), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.
