Curcumin Inhibits the Activation of Immunoglobulin E-Mediated Mast Cells and Passive Systemic Anaphylaxis in Mice by Reducing Serum Eicosanoid and Histamine Levels.
- Author:
Xian LI
1
;
Yue LU
;
Ye JIN
;
Jong Keun SON
;
Seung Ho LEE
;
Hyeun Wook CHANG
Author Information
1. College of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of Korea. hwchang@yu.ac.kr seungho@yumail.ac.kr
- Publication Type:Original Article
- Keywords:
Curcumin;
Mast cell;
Prostaglandin D2;
Leukotriene C4;
Mitogen activated protein kinase;
Passive systemic anaphylaxis
- MeSH:
Administration, Oral;
Anaphylaxis*;
Animals;
Arachidonate 5-Lipoxygenase;
Curcuma;
Curcumin*;
Cyclooxygenase 2;
Histamine*;
Immunoglobulin E;
Immunoglobulins*;
Leukotriene C4;
Mast Cells*;
Mice*;
Mitogen-Activated Protein Kinases;
Phospholipases;
Phosphorylation;
Prostaglandin D2
- From:Biomolecules & Therapeutics
2014;22(1):27-34
- CountryRepublic of Korea
- Language:English
-
Abstract:
Curcumin is naturally occurring polyphenolic compound found in turmeric and has many pharmacological activities. The present study was undertaken to evaluate anti-allergic inflammatory activity of curcumin, and to investigate its inhibitory mechanisms in immunoglobulin E (IgE)/Ag-induced mouse bone marrow-derived mast cells (BMMCs) and in a mouse model of IgE/Ag-mediated passive systemic anaphylaxis (PSA). Curcumin inhibited cyclooxygenase-2 (COX-2) dependent prostaglandin D2 (PGD2) and 5-lipoxygenase (5-LO) dependent leukotriene C4 (LTC4) generation dose-dependently in BMMCs. To probe the mechanism involved, we assessed the effects of curcumin on the phosphorylation of Syk and its downstream signal molecules. Curcumin inhibited intracellular Ca2+ influx via phospholipase Cgamma1 (PLCgamma1) activation and the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear factor-kappaB (NF-kappaB) pathway. Furthermore, the oral administration of curcumin significantly attenuated IgE/Ag-induced PSA, as determined by serum LTC4, PGD2, and histamine levels. Taken together, this study shows that curcumin offers a basis for drug development for the treatment of allergic inflammatory diseases.
