Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models.
10.4162/nrp.2016.10.3.265
- Author:
Han Ol KWON
1
;
Minhee LEE
;
Ok Kyung KIM
;
Yejin HA
;
Woojin JUN
;
Jeongmin LEE
Author Information
1. Department of Medical Nutrition, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin 17104, Korea. Jlee2007@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Chondrocyte;
Collagen;
MMP;
TIMP
- MeSH:
Administration, Oral;
Aggrecans;
Animals;
Cartilage;
Cartilage, Articular;
Cell Survival;
Chondrocytes;
Collagen;
Collagen Type I;
Collagen Type II;
Dinoprostone;
Extracellular Matrix;
In Vitro Techniques*;
Injections, Intra-Articular;
Knee Joint;
Matrix Metalloproteinases;
Models, Animal;
Nitric Oxide;
Osteoarthritis*;
Rats;
Real-Time Polymerase Chain Reaction;
Tissue Inhibitor of Metalloproteinases
- From:Nutrition Research and Practice
2016;10(3):265-273
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES: The inhibitory effect of Hijikia fusiforme (HF) extracts on degenerative osteoarthritis was examined in primary cultured rat cartilage cells and a monosodium iodoacetate (MIA)-induced osteoarthritis rat model. MATERIALS/METHODS: In vitro, cell survival and the expression of matrix metalloproteinases (MMPs), collagen type I, collagen type II, aggrecan, and tissue inhibitor of metalloproteinases (TIMPs) was measured after H2O2 (800 µM, 2 hr) treatment in primary chondrocytes. In vivo animal study, osteoarthritis was induced by intra-articular injection of MIA into knee joints of rats, and then RH500, HFE250 and HFE500 were administered orally once a day for 28 days. To determine the anti-inflammatory effects of HFE, nitric oxide (NO), prostaglandin E2 (PGE2) expression were measured. In addition, real-time PCR was performed to measure the genetic expression of MMPs, collagen type I, collagen type II, aggrecan, and TIMPs. RESULTS: In the in vitro assay, cell survival after H2O2 treatment was increased by HFE extract (20% EtOH). In addition, anabolic factors (genetic expression of collagen type I, II, and aggrecan) were increased by HFE extract (20% EtOH). However, the genetic expression of MMP-3 and 7, known as catabolic factors were significantly inhibited by treatment with HFE extract (20% EtOH). In the in vivo assay, anabolic factors (genetic expression of collagen type I, II, aggrecan, and TIMPs) were increased by oral administration of HFE extract. However, the genetic expression of MMP-3 and 7, known as catabolic factors, and production of NO and PGE2 were significantly inhibited by treatment with oral administration of HFE extract. CONCLUSIONS: HFE extract inhibited articular cartilage degeneration through preventing extracellular matrix degradation and chondrocyte injury.
