Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency.
10.14405/kjvr.2016.56.3.189
- Author:
Mi Ja PARK
1
;
Seong Joon JOH
;
Kang Seuk CHOI
;
Aeran KIM
;
Min Goo SEO
;
Jae Young SONG
;
Seon Jong YUN
Author Information
1. Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea. paru33@korea.kr
- Publication Type:Brief Communication
- Keywords:
Massachusetts 41 vaccine;
hemagglutination inhibition;
infectious bronchitis;
potency test
- MeSH:
Bronchitis;
Enzyme-Linked Immunosorbent Assay*;
Hemagglutination Inhibition Tests*;
Hemagglutination*;
Infectious bronchitis virus*;
Neutralization Tests*;
Vaccine Potency*
- From:Korean Journal of Veterinary Research
2016;56(3):189-192
- CountryRepublic of Korea
- Language:English
-
Abstract:
The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r² values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log₁₀ and HI titer of 5 log₂ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.
