Prevalence of CTX-M Extended-spectrum Beta-lactamases in Clinical Isolates of Enterobacteriaceae in Korea.
- Author:
Jungmin KIM
1
;
Yu Mi LIM
Author Information
1. Department of Microbiology, Dankook University College of Medicine, Chonan, Korea. minkim@dankook.ac.kr
- Publication Type:Original Article
- Keywords:
CTX-M-3;
CTX-M-15;
CTX-M-9;
CTX-M-14;
Enterobacteriaceae
- MeSH:
beta-Lactamases*;
Cefotaxime;
Cephalosporins;
Cloaca;
Enterobacteriaceae*;
Gram-Negative Bacteria;
Hospitals, University;
Humans;
Korea*;
Pneumonia;
Polymerase Chain Reaction;
Prevalence*
- From:Journal of Bacteriology and Virology
2004;34(4):303-310
- CountryRepublic of Korea
- Language:English
-
Abstract:
The evolution and dissemination of extended-spectrum beta-lactamases (ESBL) have compromised the clinical use of third-generation cephalosporins worldwide. Although most ESBLs belong to the TEM and SHV beta-lactamase families, the members of CTX-M, a novel ESBL family, are increasing worldwide in Gram-negative bacteria. We examined the prevalence of CTX-M ESBL in clinical isolates of the family Enterobacteriaceae collected from three university hospitals located in three different cities in Korea. Among a total of 603 isolates collected, 163 isolates (27.0%) revealed > or =2 microgram/ ml of MIC against cefotaxime, and 93 isolates (15.4%) produced ESBL confirmed by the double disk synergy test. Among 93 ESBL-producing isolates, blaCTX-M genes were detected in 41 isolates by PCR method and they included 1 isolate of C. freundii, 3 of E. aerogenes, 2 of E. cloacae, 17 of E. coli, 9 of K. pneumoniae, and 9 of S. marcescens. Thus, the overall prevalence of CTX-M ESBL-producing isolates among the family Enterobacteriaceae was 6.8% (41 of 603 isolates) and the proportion of CTX-M-producers among the ESBL-producing isolates was 44.1% (41 of 93 isolates). Further determination of the blaCTX-M subtype by nucleotide sequencing revealed blaCTX-M-3 in 17, blaCTX-M-15 in 11, blaCTX-M-14 in 9, and blaCTX-M-9 in 4 isolates. To our knowledge, this is the first report on the dissemination of CTX-M ESBL among clinical isolates of the family Enterobacteriaceae in Korea.
