The Effect of Epicatechin on the High Glucose-induced TSP-1 Expression and MMP-2 Activity in Rat Vascular Smooth Muscle Cells.
10.3803/jkes.2006.21.4.302
- Author:
Gyeong Ryul RYU
1
;
Jung Hoon KANG
;
Kyong Ja HONG
;
In Kyung JEONG
;
Hwa In JANG
;
Duck Joo RHIE
;
Shin Hee YOON
;
Sang June HAHN
;
Myung Suk KIM
;
Yang Hyeok JO
;
Myung Jun KIM
Author Information
1. Department of Physiology, College of Medicine, The Catholic University of Korea, Korea.
- Publication Type:Original Article
- Keywords:
Epicatechin;
High glucose;
Matrix metalloproteinase-2;
Thrombospondin-1;
Vascular smooth muscle cell
- MeSH:
Animals;
Antioxidants;
Aorta;
Atherosclerosis;
Blood Glucose;
Blotting, Northern;
Blotting, Western;
Catechin*;
Diabetes Mellitus, Type 2;
Gelatin;
Glucose;
Humans;
Incidence;
Matrix Metalloproteinase 2;
Muscle, Smooth, Vascular*;
Rats*;
Rats, Sprague-Dawley;
RNA, Messenger;
Thrombospondin 1*
- From:Journal of Korean Society of Endocrinology
2006;21(4):302-310
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The incidence of atherosclerosis is well correlated with the progression of type 2 diabetes mellitus. High plasma glucose in uncontrolled diabetic patients evokes many vascular complications such as atherosclerosis. Specifically, high glucose was reported to induce thrombospondin-1 (TSP-1), which activates matrix metalloproteinase-2 (MMP-2) and leads to the invasion of vascular smooth muscle cells (VSMCs) into the intima. Catechins with antioxidant effects are known to inhibit MMP-2 activity. Therefore, this study was aimed at revealing the effect of epicatechin, one of catechins, on high glucose-induced TSP-1 and the invasiveness of VSMCs. METHODS: VSMCs were primarily isolated from Sprague-Dawley rat aorta. The VSMCs were incubated with different doses (30, 100 and 300 micrometer) of epicatechin under high glucose concentration (30 mM). The TSP-1 protein and mRNA expressions were analyzed by performing Western blotting and Northern blot analyses, respectively. RT-PCR was performed to observe the MMP-2 mRNA expression. Gelatin zymography was performed for the measurement of MMP-2 activity. Invasion assays were performed to evaluate the invasiveness of VSMCs. RESULTS: Epicatechin inhibited the high glucose-induced TSP-1 expression and the MMP-2 activity in a dose-dependent manner. Also, epicatechin inhibited the high glucose-induced invasiveness of VSMCs across the matrix barrier in a dose-dependent fashion. CONCLUSION: Collectively, epicatechin may prevent the high glucose-induced proliferation and invasion of VSMCs by inhibiting the TSP-1 expression and the MMP-2 activity. Therefore, epicatechin appears to play a protective role in the development of atherosclerosis.
