A Novel Synthetic Compound 3-Amino-3-(4-Fluoro-Phenyl)-1H-Quinoline-2,4-Dione (KR22332) Exerts a Radioprotective Effect via the Inhibition of Mitochondrial Dysfunction and Generation of Reactive Oxygen Species.
10.3349/ymj.2014.55.4.886
- Author:
Seung Jae BAEK
1
;
Jae Won CHANG
;
Keun Hyung PARK
;
Garp Yeol YANG
;
Hye Sook HWANG
;
Yoon Woo KOH
;
Young Sik JUNG
;
Chul Ho KIM
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Yonsei University Wonju College of Medicine, Wonju, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Radiation induced oral mucositis;
3-amino-3-(4-fluoro-phenyl)-1H-quinoline-2,4-dione (KR22332);
apoptosis;
radioprotection
- MeSH:
Apoptosis/drug effects/physiology;
Cell Line, Tumor;
Cell Survival/drug effects/physiology;
Humans;
Keratinocytes/metabolism;
Membrane Potential, Mitochondrial/drug effects/physiology;
Radiation-Protective Agents/chemistry/*pharmacology;
Reactive Oxygen Species/metabolism
- From:Yonsei Medical Journal
2014;55(4):886-894
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Acute side effects of radiation such as oral mucositis are observed in most patients. Although several potential radioprotective agents have been proposed, no effective agent has yet been identified. In this study, we investigated the effectiveness of synthetic compound 3-amino-3-(4-fluoro-phenyl)-1H-quinoline-2,4-dione (KR22332) as a radioprotective agent. MATERIALS AND METHODS: Cell viability, apoptosis, the generation of reactive oxygen species (ROS), mitochondrial membrane potential changes, and changes in apoptosis-related signaling were examined in human keratinocyte (HaCaT). RESULTS: KR22332 inhibited irradiation-induced apoptosis and intracellular ROS generation, and it markedly attenuated the changes in mitochondrial membrane potential in primary human keratinocytes. Moreover, KR22332 significantly reduced the protein expression levels of ataxia telangiectasia mutated protein, p53, and tumor necrosis factor (TNF)-alpha compared to significant increases observed after radiation treatment. CONCLUSION: KR22332 significantly inhibited radiation-induced apoptosis in human keratinocytes in vitro, indicating that it might be a safe and effective treatment for the prevention of radiation-induced mucositis.
