Effect of Glutathione on Lead Induced Modulation of NO Synthesis in RAW 264.7 Cell.
- Author:
Sae Ron SHIN
1
;
Gyung Jae OH
;
Keun Sang KWON
;
Wook Hee YOON
Author Information
1. Department of Preventive Medicine and Public Health, Chonbuk National University Medical School and Institute for Medical Sciences, Korea. shinsr0704@hanmail.net
- Publication Type:Original Article
- Keywords:
Glutathione;
Lead;
Buthionine sulfoximine;
N-acetylcysteine;
Nitric oxide
- MeSH:
Abelson murine leukemia virus;
Acetylcysteine;
Adenosine Triphosphate;
Animals;
Buthionine Sulfoximine;
Glutathione*;
Macrophages;
Mice;
Nitric Oxide
- From:Korean Journal of Preventive Medicine
2002;35(4):269-274
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To evaluate the effect of glutathione(GSH) on lead induced modulation of nitric oxide(NO) synthesis, and to examine how lead modulates NO production in macrophages. METHODS: This study was observed in a culture of RAW 264.7 cells, which originated from a tumor in a Balb/c mouse that was induced by the Abelson murine leukemia virus. The compounds investigated were lead chloride, N-acetyl-cystein(NAC), and Buthionine Sulfoximine(BSO). RESUJLTS: ATP synthesis in RAW 264.7 cells was unchanged by each lead concentration exposure in a dose dependent manner. The NO synthesis was decreased when exposed to lead(PbCl2) concentration 0.5 micro M. The presence of 300 micro M NAC, used as a pretreatment in the culture medium, caused the recovery of the lead induced decrease in NO synthesis, but in the presence of 300 micro M BSO as a pretreatment, there was no recoverey. Pretreatment with NAC and BSO had no affect on ATP synthesis at any of the lead concentrations used. CONCLUSIONS: These results indicated that GSH has a protective effect toward lead toxicity, and suggested that the inhibition of NO production in macrophage due to lead toxicity may be related to cofactors of iNOS (inducible nitric oxide synthase)
