ICAM-1/LFA-1 interaction contributes to the induction of endothelial cell-cell separation: implication for enhanced leukocyte diapedesis.
10.3858/emm.2009.41.5.038
- Author:
Hyun WEE
1
;
Hyun Mee OH
;
Jae Hyeok JO
;
Chang Duk JUN
Author Information
1. Department of Life Science, Cell Dynamics Research Center, BioImaging Research Center, Research Center for Biomolecular Nanotechnology, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Korea. cdjun@gist.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cell movement;
endothelial cells;
intercellular adhesion molecule-1;
leukocytes;
lymphocyte function-associated antigen-1
- MeSH:
Cell Movement;
Cells, Cultured;
Endothelial Cells/*cytology/metabolism;
Flow Cytometry;
Fluorescent Antibody Technique;
Humans;
Intercellular Adhesion Molecule-1/*metabolism;
Leukocytes/cytology/*immunology;
Lymphocyte Function-Associated Antigen-1/*metabolism
- From:Experimental & Molecular Medicine
2009;41(5):341-348
- CountryRepublic of Korea
- Language:English
-
Abstract:
The basic route and mechanism for diapedesis has not yet to be fully defined. Here we present evidence that "cell-cell separation" between endothelial cells (ECs) may provide a route for leukocyte diapedesis. We unexpectedly found that extensive interaction between peripheral blood leukocytes and ECs that were activated by TNF-alpha induced the opening of EC contacts and, surprisingly, resulted in cell-cell separation. This event was specific to the intercellular adhesion molecules-1 (ICAM-1)/leukocyte function-associated antigen-1 interaction, as demonstrated by the following: (1) ICAM-1 expression correlated with increased EC contraction; and (2) the blocking of ICAM-1 selectively inhibited EC separation. Thus, we suggest that "cell-cell separation" could be a mechanism for diapedesis in situations that may require massive leukocyte infiltration.
