Identification and partial purification of pollen allergens from Artemisia princeps.
10.3349/ymj.1989.30.4.346
- Author:
Hae Sim PARK
1
;
Chein Soo HONG
;
Heung Jai CHOI
;
Kyung Soo HAHM
Author Information
1. Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Allergens;
Artemisia princeps;
electroblotting;
gel elution
- MeSH:
Blotting, Western/methods;
Electrophoresis, Agar Gel/methods;
Human;
Korea;
Lymphokines;
Plants/immunology;
Pollen/analysis/*immunology;
Skin Tests/methods
- From:Yonsei Medical Journal
1989;30(4):346-354
- CountryRepublic of Korea
- Language:English
-
Abstract:
The pollen of Artemisia has been considered as the main late summer-autumn allergen source in this country. To identify its allergenic components, Artemisia princeps pollen extracts were separated by 10% sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to nitrocellulose membrane, where IgE binding components were detected by the reaction with sera of twenty Artemisia-allergic patients and 125I-anti-human IgE, sixteen components in the molecular range of 10,000 and 85,000 daltons were detected. Twelve bands bound to IgE from 50% of the sera tested, and two bands (37,000, 23,000 daltons) showed the highest (85%) frequency of IgE-binding in twenty sera tested. When the gel of SDS-PAGE with Artemisia pollen extracts was sliced into 11 allergenic groups (AG) and the protein of each AG was obtained by the gel elution method, the wormwool-RAST inhibition test showed that the AG 10 demonstrated to be the most potent, and the AG 7 was the next. Six AGs showed significant responses (more than 100% of wheal size to histamine, 1 mg/ml) on the skin prick test in more than 50% of the patients tested. It is suggested that electrophoretic transfer analysis with SDS-PAGE may be a valuable method for Artemisia allergen identification, and the possibility of partial purification of allergens by employing gel elution is discussed.
