Corn silk induces nitric oxide synthase in murine macrophages.
- Author:
Kyung A KIM
1
;
Sang Kyu CHOI
;
Hye Seon CHOI
Author Information
1. Department of Biological Sciences the Immunomodulation Research Center University of Ulsan, Ulsan 680-749, Korea. hschoi@mail.ulsan.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
macrophages;
nitric oxide;
nitric oxide synthase;
NF-kappaB
- MeSH:
Animals;
Anticoagulants/*pharmacology;
Genistein/pharmacology;
I-kappa B/metabolism;
Macrophages/drug effects/*enzymology/metabolism;
Mice;
NF-kappa B/antagonists & inhibitors/metabolism;
Nitric Oxide/biosynthesis;
Nitric-Oxide Synthase/antagonists & inhibitors/*biosynthesis;
Phosphorylation;
Plant Extracts/pharmacology;
Polysaccharides/*pharmacology;
Protein Transport/drug effects;
Protein-Tyrosine Kinase/antagonists & inhibitors/physiology;
Pyrrolidines/pharmacology;
Research Support, Non-U.S. Gov't;
Thiocarbamates/pharmacology;
Zea mays/*chemistry
- From:Experimental & Molecular Medicine
2004;36(6):545-550
- CountryRepublic of Korea
- Language:English
-
Abstract:
Corn silk has been purified as an anticoagulant previously and the active component is a polysaccharide with a molecular mass of 135 kDa. It activates murine macrophages to induce nitric oxide synthase (NOS) and generate substantial amounts of NO in time and dose-dependent manners. It was detectable first at 15 h after stimulation by corn silk, peaked at 24 h, and undetectable by 48 h. Induction of NOS is inhibited by pyrolidine dithiocarbamate (PDTC) and genistein, an inhibitor of nuclear factor kappa B (NF-kappaB) and tyrosine kinase, respectively, indicating that iNOS stimulated by corn silk is associated with tyrosine kinase and NF-kappaB signaling pathways. IkappaB-alpha degradation was detectible at 10 min, and the level was restored at 120 min after treatment of corn silk. Corn silk induced nuclear translocation of NF-kappaB by phosphorylation and degradation of IkappaB-alpha.
