A Case of Chronic Lymphocytic Leukemia with Trisomy 12 detected by Fluorescence in situ Hybridization (FISH).
- Author:
Deok CHO
;
Won Pyo HONG
;
Hyeouug Joon KIM
;
Dong Wook RYANG
- Publication Type:Case Report
- MeSH:
Chromosomes, Human, Pair 12;
Diagnosis;
Female;
Fluorescence*;
Hepatomegaly;
HLA-DR Antigens;
Humans;
Immunophenotyping;
In Situ Hybridization*;
Interphase;
Karyotype;
Leukemia, Lymphocytic, Chronic, B-Cell*;
Lymphatic Diseases;
Lymphocytes;
Lymphocytosis;
Metaphase;
Middle Aged;
Splenomegaly;
Trisomy*
- From:Korean Journal of Clinical Pathology
1997;17(5):689-693
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We report a case of B-cell chronic lymphocytic leukemia (CLL) with trisomy 12 detected by FISH using chromosome 12 alpha-satellite Probe (Oncor , USA) in uncultured interphase cells. Chromosome studies did not produce an analyzable metaphase by standard short term culture and revealed only normal female karyotype by B cell mitogen (phorbol 12-myristate 13-acetate) stimulated 96 hr culture. The patient, a 59-year-old female, did not have hepatomegaly, splenomegaly, lymphadenopathy and any other symptoms. The peripheral blood of the patient showed marked lymphocytosis (WBC : 28,300/microL, Lymphocyte: 80%) and the diagnosis by immunophenotyping was B cell CLL:CD5, CDl9, CD2O, SmIg, HLA-DR positive.
