Anticancer effect of liposome incorporated with methotrexate and antibody against tumor specific surface antigen of rat hepatoma.
10.3349/ymj.1989.30.3.246
- Author:
Yang Sik SHIN
1
;
Eun Mee PAIK
;
Yong Ho AHN
;
Byung Soo KIM
;
Yoon Soo KIM
Author Information
1. Department of Anesthesiology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Liposome drug delivery system;
methotrexate;
tumor specific surface antigen;
hepatoma
- MeSH:
Animal;
Antibodies, Neoplasm;
Antigens, Neoplasm/*immunology;
Antigens, Surface/*immunology;
Drug Carriers;
Liposomes;
Liver Neoplasms, Experimental/*drug therapy;
Male;
Methotrexate/*administration and dosage;
Rats;
Support, Non-U.S. Gov't
- From:Yonsei Medical Journal
1989;30(3):246-255
- CountryRepublic of Korea
- Language:English
-
Abstract:
antibody against tumor specific surface membrane protein was produced by immunizing a New Zealand White rabbit with antigen (66 kDa) prepared from the plasma membrane of rat hepatoma induced by feeding a diet containing 3'-methyl-4-dimethylaminoazobenzene, and was purified by protein A-Sepharose 6MB affinity chromatography. The purified antibody was incorporated into liposomes by a reverse phase evaporation vesicle method in order to prepare a tumor specific anticancer drug carrier. The effect of the antibody against tumor specific antigen was evaluated by comparing the inhibition of DNA synthesis in hepatoma cells with different preparations of methotrexate. Methotrexate encapsulated into liposome showed a stronger inhibitory effect on DNA synthesis (1.4-1.7 times) than free methotrexate. Liposomes having the antibody showed stronger inhibitory effect (3.1 times) on DNA synthesis than free methotrexate group in hepatic nodular area. From these results, it is concluded that tumor specific antibody inserted into liposomal membrane would be recognized by surface antigens which were expressed on the plasma surface membrane of rat hepatoma cells and thereby increase the carrying efficiency of drugs to the target cells. This could be useful in cancer chemotherapy.
