The Effects of Substrates and Hydrostatic Pressure on Differentiation of Mesenchymal Stem Cell.
- Author:
Ki Chan AN
1
;
Dae Hyun PARK
;
Jae Sang CHOI
;
Gyu Min KONG
;
Joo Yong KIM
;
Byung Ho HA
;
Jung Wook SHIN
;
So Hee PARK
;
Yun Gyeong KANG
Author Information
1. Department of Orthopedic Surgery, College of Medicine,Inje University, Busan Paik Hospital, Busan, Korea. 97111033@hanmail.net
- Publication Type:Original Article
- Keywords:
Osteochondral defects;
Poly (epsilon-caprolactone);
Alginate;
Mesenchymal stem cells;
Intermittent hydrostatic pressure (IHP)
- MeSH:
Bone and Bones;
Cartilage;
Cartilage, Articular;
Chondrocytes;
Cues;
DNA;
Fluorescent Antibody Technique;
Hydrostatic Pressure*;
Intercellular Signaling Peptides and Proteins;
Mesenchymal Stromal Cells*;
Osteoblasts;
Stem Cells;
Syringes;
Tissue Engineering
- From:Journal of Korean Orthopaedic Research Society
2014;17(1):1-12
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study investigated the potential of dual differentiation of stem cells into osteo- and chodrogenesis depending on scaffold type even in the same environment. MATERIALS AND METHODS: For the part of the cartilage tissue section, MSCs were suspended in alginate solution and bead droplets were made using 23G syringe. For the bone tissue section, PCL/HA scaffolds were made using the bio-plotting system followed by seeding mesenchymal stem cells (MSCs) onto the scaffolds. Scaffolds with MSCs were cultured in cocktail media containing osteogenic and chondrogenic growth factors for up to 21 days. To provide mechanical environments which articular cartilage experiences in-vivo, intermittent hydrostatic pressure (IHP) was engaged. Various cellular responses were assessed: the quantitative analysis of DNA contents, GAG contents, ALP activities and immunofluorescence. RESULTS: We found that IHP promoted MSCs differentiation into the targeted cell types. That is, MSCs in alginate scaffolds were able to be differentiated into chondrocytes, while those onto PCL/HA scaffolds were able to be differentiated into osteoblasts. CONCLUSION: Depending on the scaffold characteristics MSCs can be differentiated into bone cells or chondrocytes. This technique can provide a cue for the treatment of osteochondral defects utilizing tissue engineering.
