Detailed Characterization of Chromosomal Breakpoints in an ALL, L3 using Cross Species Color Banding.
- Author:
Young Mi JEON
1
;
Chun Hwa IHM
;
Moon Hee KIM
;
Sun Hoe KOO
;
Jong Woo PARK
;
Kye Chul KWON
Author Information
1. Department of Clinical Pathology Chungnam National University, Taejeon, Korea.
- Publication Type:Original Article
- Keywords:
Cross-pecies color banding (RxFISH);
G-anding, ALL (L3)
- MeSH:
Chromosome Aberrations;
Chromosomes, Human;
Cytogenetics;
Hominidae;
Humans;
Karyotype;
Leukemia;
Mass Screening
- From:Korean Journal of Clinical Pathology
2000;20(5):516-519
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Karyotype analysis by G-anding is the standard method for identifying numerical and structural chromosomal aberrations in cytogenetic laboratories. However, the origins of marker chromosomes, subtle translocations, or complex chromosomal rearrangements are often difficult to identify with certainty. Cross-pecies color banding is a new FISH-ased screening technique that enables the generation of a specific color banding pattern for each human chromosome based on the genomic homologies between man and various species of apes. We report application of Cross-pecies color banding (RxFISH) to characterize the chromosomal rearrangements of one leukemia sample the G-and karyotype of which were incomplete. The combination of G-anding and RxFISH in this case study yielded additional information beyond that obtained by either technique used alone in determining the precise breakpoints in complex chromosomal rearrangements.
