The Effects of Antihypertensive Drugs on Bone Mineral Density in Ovariectomized Mice.
10.3346/jkms.2013.28.8.1139
- Author:
Kwi Young KANG
1
;
Yoongoo KANG
;
Mirinae KIM
;
Youngkyun KIM
;
Hyoju YI
;
Juryun KIM
;
Hae Rin JUNG
;
Sung Hwan PARK
;
Ho Youn KIM
;
Ji Hyeon JU
;
Yeon Sik HONG
Author Information
1. Division of Rheumatology, Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea. rhcow1@catholic.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Antihypertensive Agents;
Osteoporosis;
Ovariectomized Mice;
Thiazides;
Telmisartan
- MeSH:
Animals;
Antihypertensive Agents/*pharmacology;
Atrophy;
Benzimidazoles/pharmacology;
Benzoates/pharmacology;
Bone Density/*drug effects;
Enalapril/pharmacology;
Female;
Mice;
Mice, Inbred C57BL;
Ovariectomy;
Propranolol/pharmacology;
Thiazides/pharmacology;
Tibia/radiography;
Tomography, X-Ray Computed;
Uterus/anatomy & histology/pathology
- From:Journal of Korean Medical Science
2013;28(8):1139-1144
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effects of several antihypertensive drugs on bone mineral density (BMD) and micro-architectural changes in ovariectomized (OVX) mice were investigated. Eight-week-old female C57/BL6 mice were used for this study. Three days after ovariectomy, mice were treated intraperitoneally with nifedipine (15 mg/kg), telmisartan (5 mg/kg), enalapril (20 mg/kg), propranolol (1 mg/kg) or hydrochlorothiazide (12.5 mg/kg) for 35 consecutive days. Uterine atrophy of all mice was confirmed to evaluate estrogen deficiency state. BMD and micro-architectural analyses were performed on tibial proximal ends by micro-computed tomography (micro-CT). When OVX mice with uterine atrophy were compared with mice without atrophy, BMD decreased (P < 0.001). There were significant differences in BMD loss between different antihypertensive drugs (P = 0.005). Enalapril and propranolol increased BMD loss in mice with atrophied uteri compared with control mice. By contrast, thiazide increased BMD in mice with uterine atrophy compared with vehicle-treated mice (P = 0.048). Thiazide (P = 0.032) and telmisartan (P = 0.051) reduced bone loss and bone fraction in mice with uterine atrophy compared with the control. Thiazide affects BMD in OVX mice positively. The reduction in bone loss by thiazide and telmisartan suggest that these drugs may benefit menopausal women with hypertension and osteoporosis.
