Viral Etiologic Agents in Acute Viral Lower Respiratory Tract Detected by Multiplex RT-PCR.
- Author:
Hee Yeong CHEONG
1
;
Jung Ho LEE
;
Yong Bae KIM
;
Hae Son NAM
;
Young Jin CHOI
;
Chang Jin KIM
;
Joon Soo PARK
Author Information
1. Department of Pediatrics, Soonchunhyang University Hospital, Soonchunhyang University School, Chungnam, Korea. allergist@korea.com
- Publication Type:Original Article
- Keywords:
Viral infection;
Lower respiratory tract infection;
Clinical manifestations
- MeSH:
Adenoviridae;
Bronchiolitis;
Child;
Child, Hospitalized;
Chungcheongnam-do;
Cough;
Croup;
Diagnosis;
Fever;
Humans;
Orthomyxoviridae;
Paramyxoviridae Infections;
Pneumonia;
Respiratory Sounds;
Respiratory Syncytial Viruses;
Respiratory System*;
Respiratory Tract Infections;
Seasons
- From:Pediatric Allergy and Respiratory Disease
2007;17(4):334-353
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE:This study was performed to examine seasonal occurrences of respiratory viruses and detect viral etiologic agents, by multiplex RT-PCR in children with acute lower respiratory tract infection (ALRI). METHODS:Nasopharyngeal aspirates (NPA) were collected from 654 hospitalized children with ALRI on admission at Soonchunhyang University Cheonan Hospital and analysed by multiplex RT-PCR between January 2005 and December 2005. RESULTS:Viral agents were detected from 234 subjects (35.8%). The identified viral pathogens were respiratory syncytial virus (RSV) (34.2%), adenovirus (33.8%), parainfluenza virus (21.8%), influenza virus type A (8.1%) and influenza virus type B (2.1%). Viral ALRI occurred most frenquently during the 1st year of life. Clinical diagnoses of viral ALRI included pneumonia (72.2%), bronchiolitis (14.5%), tracheobronchitis (9.8%) and croup (3.5%). The most common cause of bronchiolitis was RSV. Common clinical symptoms and signs of viral ALRI were cough (93.4%), fever (78.2%), rhinorrhea (72.5%), rale (60.2%), and wheezing (26.2%). CONCLUSION:Viral agents were detected by multiplex RT-PCR in 35.8% of NPA obtained from 654 hospitalized patients with acute respiratory tract infections. Multiplex RT-PCR may be useful for detecting virus in children.
