Synergistic effect of peroxiredoxin II antisense on cisplatin-induced cell death.
- Author:
Young Do YOO
1
;
Young Min CHUNG
;
Jong Kuk PARK
;
Chul Min AHN
;
Sung Kyu KIM
;
Hyung Jung KIM
Author Information
1. Korea University Cancer Institute, Korea University College of Medicine, Seoul, Korea. khj57@yumc.yonsei.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Prx II;
cisplatin;
chemosensitizer;
antisense;
multidrug resistance
- MeSH:
Antineoplastic Agents/*pharmacology;
Antioxidants/metabolism;
Apoptosis/drug effects;
Cell Death/*drug effects;
Cisplatin/*pharmacology;
Dose-Response Relationship, Drug;
Drug Synergism;
Genetic Vectors;
Human;
Oligonucleotides, Antisense/*metabolism;
Peroxidases/*metabolism;
Plasmids/genetics/metabolism;
Stomach Neoplasms/*metabolism;
Tumor Cells, Cultured
- From:Experimental & Molecular Medicine
2002;34(4):273-277
- CountryRepublic of Korea
- Language:English
-
Abstract:
Peroxiredoxin II (Prx II) is known not only to protect cells from oxidative damage caused by hydrogen peroxide (H2O2), but also to endow cancer cells with resistance to both H2O2 and cisplatin and to grant them radioresistance. In this study, we examined whether Prx II antisense could enhance cisplatin-induced cell death. When gastric cancer cells were transfected with various concentrations of Prx II antisense plasmid, pPrxII/AS, and then treated with the same concentrations of cisplatin, Prx II antisense enhanced cisplatin-induced cell death. The combination index (CI) at all doses of the combination was below 1, indicating that Prx II antisense sensitized cisplatin-induced cell death. This synergism was also observed in the cells transfected with a Prx II antisense oligomer. Our present results, therefore, suggest that Prx II antisense would be a very good sensitizer for cisplatin, and that Prx II as a target for chemosensitizers constitutes a promising avenue for future research.
