Cotransplanted Bone Marrow Derived Mesenchymal Stem Cells (MSC) Enhanced Engraftment of Hematopoietic Stem Cells in a MSC-dose Dependent Manner in NOD/SCID Mice.
10.3346/jkms.2006.21.6.1000
- Author:
Dong Hyun KIM
1
;
Keon Hee YOO
;
Young Sook YIM
;
Jaewon CHOI
;
Soo Hyun LEE
;
Hye Lim JUNG
;
Ki Woong SUNG
;
Sung Eun YANG
;
Won Il OH
;
Yoon Sun YANG
;
Sang Hee KIM
;
Sang Yun CHOI
;
Hong Hoe KOO
Author Information
1. School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Hematopoietic Stem Cells;
Mesenchymal Stem Cells;
Transplantation;
Mice, SCID;
Engraftment
- MeSH:
Middle Aged;
Mice, SCID;
Mice, Inbred NOD;
Mice;
Mesenchymal Stem Cells/*cytology;
Mesenchymal Stem Cell Transplantation/*methods;
Humans;
Hematopoietic Stem Cells/*cytology;
Hematopoietic Stem Cell Transplantation/*methods;
Graft Survival/*physiology;
Cells, Cultured;
Cell Count;
Animals;
Adult
- From:Journal of Korean Medical Science
2006;21(6):1000-1004
- CountryRepublic of Korea
- Language:English
-
Abstract:
Transplantation of marrow-derived mesenchymal stem cells (MSCs), expanded by culture in addition to whole bone marrow, has been shown to enhance engraftment of human hematopoietic stem cells (HSCs). Our hypothesis was that there might be an optimum ratio range that could enhance engraftment. We examined the percent donor chimerism according to the ratio of HSCs to MSCs in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. We tested a series of ratios of co-transplanted CD34+-selected bone marrow cells, and marrow-derived MSCs into sublethally irradiated NOD/SCID mice. In all experiments, 1 x 10(5) bone marrow derived human CD34+ cells were administered to each mouse and human MSCs from different donors were infused concomitantly. We repeated the procedure three times and evaluated engraftment with flow cytometry four weeks after each transplantation. Serial ratios of HSCs to MSCs were 1:0, 1:1, 1:2 and 1:4, in the first experiment, 1:0, 1:1, 1:2, 1:4 and 1:8 in the second and 1:0, 1:1, 1:4, 1:8 and 1:16 in the third. Cotransplantation of HSCs and MSCs enhanced engraftment as the dose of MSCs increased. Our results suggest that the optimal ratio of HSCs and MSCs for cotransplantation might be in the range of 1:8-1:16; whereas, an excessive dose of MSCs might decrease engraftment efficiency.
