Clarithromycin Susceptibility Testing of Mycobacterium avium Complex Using 2,3-Diphenyl-5-thienyl-(2)-tetrazolium Chloride Microplate Assay with Middlebrook 7H9 Broth.
10.3346/jkms.2009.24.3.511
- Author:
Young Kil PARK
1
;
Won Jung KOH
;
Shin Ok KIM
;
Sonya SHIN
;
Bum Joon KIM
;
Sang Nae CHO
;
Sun Min LEE
;
Chulhun L CHANG
Author Information
1. Korean Institute of Tuberculosis, Seoul, Korea.
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Tetrazolium;
Microtiter Method;
Mycobacterium Avium Complex;
Clarithromycin;
Susceptibility Testing
- MeSH:
Clarithromycin/*pharmacology;
Culture Media;
Humans;
Microbial Sensitivity Tests/*methods;
Mycobacterium avium Complex/*drug effects/isolation & purification;
Tetrazolium Salts/*chemistry
- From:Journal of Korean Medical Science
2009;24(3):511-512
- CountryRepublic of Korea
- Language:English
-
Abstract:
A series of 119 Mycobacterium avium complex isolates were subjected to clarithromycin susceptibility testing using microplates containing 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC). Among 119 isolates, 114 (95.8%) were susceptible to clarithromycin and 5 were resistant according to the new and the standard method. STC counts the low cost and reduces the number of procedures needed for susceptibility testing.
