In Vitro Release of L-dopa and Dopamine by Genetically Modified Fibroblasts of the Rat.
- Author:
Bong Sub CHUNG
1
Author Information
1. Department of Neurosurgery, Hallym University, College of Medicine, Kang Dong Sung Shim Hospital, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Tyrosine hydroxylase;
L-dopa decarboxylase;
Fibroblast;
Gene transfer;
Parkinson disease
- MeSH:
Animals;
Calcium;
Cell Culture Techniques;
Chromatography, Liquid;
Clone Cells;
DNA;
DNA, Complementary;
Dopamine*;
Fibroblasts*;
Genetic Therapy;
Levodopa*;
Parkinson Disease;
Rats*;
Transfection;
Tyrosine;
Tyrosine 3-Monooxygenase
- From:Journal of Korean Neurosurgical Society
1993;22(4):529-536
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
A construct DNA containing the cDNA for rat tyrosine hydroxylase(TH) and another construct DNA containing the cDNA for bovine L-dopa decarboxylase(DDC) were made and those genes were transferred by calcium phosphate transfection method into the immortalized rat fibroblasts. A both TH- & DDC-positive clone was identified by immunocytochemical staining. These cells produced L-dopa and dopamine and released them into the cell culture medium in vitro, which was confirmed by high performance liquid chromatography. These results have general implications for the application of gene therapy and specific implications for Parkinson disease.
