T Cell Chemokine Inducibility of Lipopolysaccharide, Interleukin (IL)-12/IL-2 and Interferon Gamma in Mouse Brain.
- Author:
Ji Eun KIM
1
;
Dong Kuck LEE
;
Seong Il SUH
;
Won Ki BAEK
;
Min Ho SUH
;
Taeg Kyu KWON
;
Jong Wook PARK
;
Chung Kyu SUH
Author Information
1. Department of Neurology, School of Medicine, Catholic University of Daegu.
- Publication Type:Original Article
- Keywords:
Chemokine;
Lipopolysaccharide;
Interleukin-12;
Interleukin-2;
Interferon gamma;
Brain
- MeSH:
Animals;
Brain Diseases;
Brain*;
Chemokines;
Gene Expression;
Humans;
Interferons*;
Interleukin-12;
Interleukin-2;
Interleukins*;
Mice*
- From:Journal of the Korean Neurological Association
2001;19(2):155-162
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Interferon gamma (IFN-gamma)-inducible protein 10 (IP-10) and monokine induced by IFN-gamma(Mig) are members of CXC-chemokine family, that are produced from macrophage/monocyte activated by IFN-gamma. These chemokines especially recruit activated T cell into inflammatory site. Here, we studied effect of lipopolysaccharide (LPS), interleukin (IL)-12/IL-2 or IFN-gammaon induction of MIG and IP-10 in mouse brain. METHODS: In order to evaluate Mig and IP-10 gene expression in brain, we injected LPS, IFN-gammaor IL-12/IL-2 into mice intraperitoneally, and measured chemokine message in brain by RT-PCR. RESULTS: In vivo injection of LPS induced Mig and IP-10 gene expression in brain of normal mice and IFN-gammaknockout mouse, however, in vivo injection of IL-12/IL-2 induced Mig and IP-10 gene expression in only the brain of normal mice. IFN-gammainduced chemokine expression in cultured brain cells, but anti-inflammatory drugs did not block IFN-gammaeffects. CONCLUSIONS: Immune stimulating agents, LPS or IL-12/IL-2 or IFN-gamma, can induce T cell chemokine gene expression in brain cells, and these chemokines may play a role in T cell infiltration in various brain diseases. (J Korean Neurol Assoc 19(2):155~162, 2001)
