Antiplasmodial and Cytotoxic Activities of Toad Venoms from Southern Amazon, Brazil.
10.3347/kjp.2016.54.4.415
- Author:
Felipe Finger BANFI
1
;
Karla de Sena GUEDES
;
Carla Regina ANDRIGHETTI
;
Ana Carolina AGUIAR
;
Bryan Wender DEBIASI
;
Janaina da Costa NORONHA
;
Domingos de Jesus RODRIGUES
;
Gerardo Magela Vieira JÚNIOR
;
Bruno Antonio Marinho SANCHEZ
Author Information
1. Universidade Federal de Mato Grosso, Instituto de Ciências da Saúde, Sinop, MT, Brazil. bamsanchez@hotmail.com
- Publication Type:Original Article
- Keywords:
Plasmodium falciparum;
antiplasmodial effect;
toad venom;
bufadienolide;
malaria chemotherapy
- MeSH:
Amphibian Venoms*;
Animals;
Biodiversity;
Brazil*;
Bufo marinus;
Malaria;
Neutral Red;
Parasites;
Plasmodium falciparum
- From:The Korean Journal of Parasitology
2016;54(4):415-421
- CountryRepublic of Korea
- Language:English
-
Abstract:
The drug-resistance of malaria parasites is the main problem in the disease control. The huge Brazilian biodiversity promotes the search for new compounds, where the animal kingdom is proving to be a promising source of bioactive compounds. The main objective of this study was to evaluate the antiplasmodial and cytotoxic activity of the compounds obtained from the toad venoms of Brazilian Amazon. Toad venoms were collected from the secretion of Rhinella marina and Rhaebo guttatus in Mato Grosso State, Brazil. The powder was extracted at room temperature, yielding 2 extracts (RG and RM) and a substance ('1') identified as a bufadienolide, named telocinobufagin. Growth inhibition, intraerythrocytic development, and parasite morphology were evaluated in culture by microscopic observations of Giemsa-stained thin blood films. Cytotoxicity was determined against HepG2 and BGM cells by MTT and neutral red assays. The 2 extracts and the pure substance ('1') tested were active against chloroquine-resistant Plasmodium falciparum strain, demonstrating lower IC₅₀ values. In cytotoxic tests, the 2 extracts and substance '1' showed pronounced lethal effects on chloroquine-resistant P. faciparum strain and low cytotoxic effect, highlighting toad parotoid gland secretions as a promising source of novel lead antiplasmodial compounds.
